Contact

Dr. Yusuke Murayama

Address: Spemannstr. 38
72076 Tübingen
Room number: 253
Phone: +49 7071 601 663
Fax: +49 7071 601 652
E-Mail: yusuke.murayama

 

Picture of Murayama, Yusuke, Dr.

Yusuke Murayama

Position: Research Scientist  Unit: Logothetis

Introduction

In our efforts study the brain simultaneously at different spatiotemporal scales we have long been developing and optimizing two powerful methodologies. These are first, in vivo connectivity based on MR-detectable paramagnetic transsynaptic tracers, such as manganese, e.g.(Saleem et al., 2002, Murayama et al., 2006, Eschenko et al., 2010), and second, direct electrical stimulation and fMRI combined (DES-fMRI) (Tolias et al., 2005, Logothetis et al., 2010). The latter permits the investigation of many different large-scale interactions, including the organization of projective fields, large-scale effects of local synaptic plasticity changes, and the spatiotemporal profile of neuromodulatory effects induced by diffuse ascending systems. Yet, our most recent pilot physiology-MRI study examining global signal propagation following electrical stimulation has demonstrated that DES most likely silences all cortical areas receiving direct input from the fibers that they excite (Logothetis et al., 2010). Together with results from previous electrophysiology studies, this finding is thought to reflect the synaptic organization of microcircuits and their response to an unnatural spatiotemporal input organization. Specifically, optimal operation of recurrent microcircuits is only ensured with spatio-temporally structured input patterns that respect synaptic delays. ES violates this principle and consequently silences the output of neocortex. To better understand feedforward signal propagation for spatiotemporally blurred inputs, we have conducted two more series of experiments.

The first study aimed to examine whether unstructured whole-field visual stimulation may to some extent resemble the pattern of signal. We used whole-field luminance flicker as stimulus and recorded from areas V1 and V2 during EPI MR imaging. In the second study we have started a systematic mapping of thalamus by local microstimulation and imaging. The aim of this investigation is to map cortico-subcortico-cortical pathways by means of ortho- and antidromic stimulation and try to understand the logic of the replication principle, namely the existence of a cortico-thalamo-cortical connection for any given cortico-cortical connection of two points, each in one cortical area. We stimulate the pulvinar but we also record spontaneous activity and study locally generated characteristic events that may be related to thalamo-cortical communication.

 

Methods

Recording and microstimulation hardware, including electrodes and microdrives, were developed at the Max Planck Institute for Biological Cybernetics. Recording chambers and head holders were positioned stereotaxically on the basis of individual, high-resolution MRI. Stimulation sites were selected so as to ensure reliable electrically evoked BOLD activation on the operculum of the brain. Visual stimuli (structured stimuli or whole-field luminance flicker) were presented binocularly, initially with our own custom-made MR-compatible visual stimulator. Electrical microstimulation was performed with a custom-built constant current source. Current amplitude, pulse duration, train duration and stimulation frequency were controlled digitally using our own TCL-based software and a QNX (Canada) real time operating system.

 

Results and Initial Conclusions

The stimulation results were presented in detail in a recent publication (Logothetis et al., 2010). The response to luminance flicker was surprising indeed. Figure 1 shows the maps and Figure 2 the temporal dynamic of the BOLD and neural responses to two types of stimulation: structured (counterphase pinwheel) and full-field luminance flicker. Striking and consistence was the patent deactivation of V2. V1 showed PBR in some animal and NBR in others. Physiological responses often showed continuation of stimulus-induced modulation, albeit with a strongly reduced baseline activity. It follows that unstructured, full-field luminance stimulations, just like the electrical stimulation, blurs the normal spatiotemporal input patterns in cortex and strong disrupts signal propagation. Our current experiments in thalamus yielded the first patterns of cortical activation (work in progress). Characteristic is the widespread activation that is expected given the parallel projections of this nucleus to the entire cortex.

 

BOLD responses to counter-phase and Full-Field Luminance Flicker

Figure 1: BOLD responses to counter-phase and Full-Field Luminance Flicker. The left plots shows response to counterphase flicker. As expected there is strong V1 and V2 activation accompanied by sustained negative BOLD (NBR) in non-stimulated areas.

 

Time course of BOLD and of neural responses to counter-phase and Full-Field Luminance Flicker.

Figure 2: Time course of BOLD and of neural responses to counter-phase and Full-Field Luminance Flicker. Top row shows responses to structured flicker. Red and Blue curves are BOLD responses in V1 and V2 respectively. Peristimulus histograms show multiunit responses in V1 (left) and V2 (right). Bottom row: same conventions but for full-field luminance flicker.

 

 

Collaborations

In the aforementioned studies I have been collaborating with Mark Augath, Josina Goense and Hellmut Merkle on MR in MR imaging, with Alexander Rauch and Nikos K. Logothetis in neuroscientific matters and data analysis, and with Axel Oeltermann in developing all the hardware required to perform the experiments (see details in publications).

 

References

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Articles (30):

Kaplan R, Adhikari MH, Hindriks R, Mantini D, Murayama Y, Logothetis NK and Deco G (March-2016) Hippocampal Sharp-Wave Ripples Influence Selective Activation of the Default Mode Network Current Biology 26(5) 686–691.
Hindriks R, Adhikari MH, Murayama Y, Ganzetti M, Mantini D, Logothetis NK and Deco G (February-2016) Can sliding-window correlations reveal dynamic functional connectivity in resting-state fMRI? NeuroImage 127 242–256.
Bahmani H, Murayama Y, Logothetis NK and Keliris GA (September-2014) Binocular Flash Suppression in the Primary Visual Cortex of Anesthetized and Awake Macaques PLoS ONE 9(9) 1-8.
Watanabe M, Bartels A, Macke JH, Murayama Y and Logothetis NK (November-2013) Temporal Jitter of the BOLD Signal Reveals a Reliable Initial Dip and Improved Spatial Resolution Current Biology 23(21) 2146–2150.
Logothetis NK, Eschenko O, Murayama Y, Augath M, Steudel T, Evrard HC, Besserve M and Oeltermann A (November-2012) Hippocampal-cortical interaction during periods of subcortical silence Nature 491(7425) 547–553.
Biessmann F, Murayama Y, Logothetis NK, Müller KR and Meinecke FC (July-2012) Improved decoding of neural activity from fMRI signals using non-separable spatiotemporal deconvolutions NeuroImage 61(4) 1031–1042.
Sultan F, Augath M, Hamodeh S, Murayama Y, Oeltermann A, Rauch A and Thier P (June-2012) Unravelling cerebellar pathways with high temporal precision targeting motor and extensive sensory and parietal networks Nature Communications 3(924) 1-10.
Eschenko O, Evrard HC, Neves RM, Beyerlein M, Murayama Y and Logothetis NK (February-2012) Tracing of noradrenergic projections using manganese-enhanced MRI NeuroImage 59(4) 3252–3265.
Magri C, Schridde U, Murayama Y, Panzeri S and Logothetis NK (January-2012) The Amplitude and Timing of the BOLD Signal Reflects the Relationship between Local Field Potential Power at Different Frequencies Journal of Neuroscience 32(4) 1395-1407.
Murayama Y, Augath M and Logothetis NK (December-2011) Activation of SC during electrical stimulation of LGN: retinal antidromic stimulation or corticocollicular activation? Magnetic Resonance Imaging 29(10) 1351-1357.
Watanabe M, Cheng K, Murayama Y, Ueno K, Asamizuya T, Tanaka K and Logothetis NK (November-2011) Attention But Not Awareness Modulates the BOLD Signal in the Human V1 During Binocular Suppression Science 334(6057) 829-831.
Mamedov I, Canals S, Henig J, Beyerlein M, Murayama Y, Mayer HA, Logothetis NK and Angelovski G (December-2010) In Vivo Characterization of a Smart MRI Agent That Displays an Inverse Response to Calcium Concentration ACS Chemical Neuroscience 1(12) 819-828.
Sultan F, Hamodeh S, Murayama Y, Saleem KS and Logothetis NK (October-2010) Flat map areal topography in Macaca mulatta based on combined MRI and histology Magnetic Resonance Imaging 28(8) 1159-1164.
Murayama Y, Biessmann F, Meinecke FC, Müller K-R, Augath M, Oeltermann A and Logothetis NK (October-2010) Relationship between neural and hemodynamic signals during spontaneous activity studied with temporal kernel CCA Magnetic Resonance Imaging 28(8) 1095-1103.
Logothetis NK, Augath M, Murayama Y, Rauch A, Sultan F, Goense J, Oeltermann A and Merkle H (October-2010) The effects of electrical microstimulation on cortical signal propagation Nature Neuroscience 13(10) 1283-1291.
Eschenko O, Canals S, Simanova I, Beyerlein M, Murayama Y and Logothetis NK (February-2010) Mapping of functional brain activity in freely behaving rats during voluntary running using manganese-enhanced MRI: Implication for longitudinal studies Neuroimage 49(3) 2544-2555.
Logothetis NK, Murayama Y, Augath M, Steffen T, Werner J and Oeltermann A (May-2009) How not to study spontaneous activity NeuroImage 45(4) 1080-1089.
Canals S, Beyerlein M, Murayama Y and Logothetis NK (September-2008) Electric stimulation fMRI of the perforant pathway to the rat hippocampus Magnetic Resonance Imaging 26(7) 978-986.
Belitski A, Gretton A, Magri C, Murayama Y, Montemurro MA, Logothetis NK and Panzeri S (May-2008) Low-frequency Local Field Potentials and Spikes in Primary Visual Cortex Convey Independent Visual Information Journal of Neuroscience 28(22) 5696-5709.
Canals S, Beyerlein M, Keller AL, Murayama Y and Logothetis NK (April-2008) Magnetic Resonance Imaging of Cortical Connectivity in vivo Neuroimage 40(2) 458-472.
Rasch MJ, Gretton A, Murayama Y, Maass W and Logothetis NK (March-2008) Inferring Spike Trains From Local Field Potentials Journal of Neurophysiology 99(3) 1461-1476.
Montemurro MA, Rasch MJ, Murayama Y, Logothetis NK and Panzeri S (March-2008) Phase-of-Firing Coding of Natural Visual Stimuli in Primary Visual Cortex Current Biology 18(5) 375-380.
Gretton A, Belitski A, Murayama Y, Schölkopf B and Logothetis NK (April-2006) The Effect of Artifacts on Dependence Measurement in fMRI Magnetic Resonance Imaging 24(4) 401-409.
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Murayama Y, Weber B, Kadharbatcha SS, Augath M and Logothetis NK (March-2006) Tracing neural circuits in vivo with Mn-enhanced MRI Magnetic Resonance Imaging 24(4) 349-358.
Leopold DA, Murayama Y and Logothetis NK (April-2003) Very Slow Activity Fluctuations in Monkey Visual Cortex: Implications for Functional Brain Imaging Cerebral Cortex 13(4) 422-433.
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Wang Y, Fujita I and Murayama Y (March-2003) Coding of visual patterns and textures in monkey inferior temporal cortex NeuroReport 14(3) 453-457.
Wang Y, Fujita I, Tamura H and Murayama Y (January-2002) Contribution of GABAergic Inhibition to Receptive Field Structures of Monkey Inferior Temporal Neurons Cerebral Cortex 12(1) 62-74.
Wang Y, Fujita I and Murayama Y (August-2000) Neuronal mechanisms of selectivity for object features revealed by blocking inhibition in inferotemporal cortex Nature Neuroscience 3(8) 807-813.
Murayama Y, Fujita I and Kato M (April-1997) Contrasting forms of synaptic plasticity in monkey inferotemporal and primary visual cortices NeuroReport 8(6) 1503–1507.
Oda Y, Charpier S, Murayama Y, Suma C and Korn H (September-1995) Long-term potentiation of glycinergic inhibitory synaptic transmission Journal of Neurophysiology 74(3) 1056-1074.

Conference papers (3):

Biessmann F, Murayama Y, Logothetis NK, Müller K-R and Meinecke FC (2012) Non-separable Spatiotemporal Brain Hemodynamics Contain Neural Information In: Machine Learning and Interpretation in Neuroimaging, , NIPS Workshop on Machine Learning and Interpretation in Neuroimaging (MLINI 2011), Springer, Berlin, Germany, 140-147, Series: Lecture Notes in Computer Science ; 7263.
Sultan F, Augath M, Murayama Y, Tolias AS and Logothetis NK (December-2011) esfMRI of the upper STS: further evidence for the lack of electrically induced polysynaptic propagation of activity in the neocortex, 9th Workshop of the International School on Magnetic Resonance and Brain Function, Magnetic Resonance Imaging, 29(10), 1374-1381.
Gretton A, Smola AJ, Bousquet O, Herbrich R, Belitski A, Augath M, Murayama Y, Pauls J, Schölkopf B and Logothetis NK (January-2005) Kernel Constrained Covariance for Dependence Measurement, Tenth International Workshop on Artificial Intelligence and Statistics (AISTATS 2005), Society for Artificial Intelligence and Statistics, Fort Lauderdale, FL, USA, 112-119.
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Posters (36):

Smuda J, Klein C, Murayama Y, Steudel T, Krampe E, Oeltermann A, Werner J, Logothetis NK and Evrard HC (November-14-2017): Microstimulation of the anterior insular cortex in the macaque monkey, 47th Annual Meeting of the Society for Neuroscience (Neuroscience 2017), Washington, DC, USA.
Klein C, Smuda J, Murayama Y, Steudel T, Krampe E, Oeltermann A, Werner J, Logothetis NK and Evrard HC (November-14-2017): NET-fMRI examination of the relation between the anterior insula and whole-brain activity in the macaque monkey, 47th Annual Meeting of the Society for Neuroscience (Neuroscience 2017), Washington, DC, USA.
Smuda J, Klein CA, Murayama Y, Steudel T, Krampe E, Oeltermann A, Werner J, Logothetis NK and Evrard H (May-22-2017): Local field potential activity in the macaque anterior insular cortex, 12th National Congress of the Belgian Society for Neuroscience (BSN 2017), Ghent, Belgium, Frontiers in Neuroscience, Conference Abstract: 12th National Congress of the Belgian Society for Neuroscience.
Balla DZ, Merkle H, Hennel F, Steudel T, Murayama Y, Pohmann R, Scheffler K and Logothetis NK (September-29-2016): Fast fMRI in nonhuman primates at 4.7T with multiband EPI and a 4 Tx/Rx + 1 Rx phased-array concept, 33rd Annual Scientific Meeting of the European Society for Magnetic Resonance in Medicine and Biology (ESMRMB 2016), Wien, Austria.
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Kaplan R, Adhikari M, Hindriks R, Mantini D, Murayama Y, Logothetis NK and Deco G (February-25-2016): Hippocampal sharp-wave ripples influence selective activation of the default mode network, Computational and Systems Neuroscience Meeting (COSYNE 2016), Salt Lake City, UT, USA.
Balla DZ, Pohmann R, Shajan G, Ehses P, Nauerth A, Steudel T, Murayama Y, Oeltermann A, Munk MH, Merkle H, Beyerlein M, Evrard HC, Logothetis NK and Scheffler K (June-2-2015): 500 ms temporal and 750 μm spatial inplane resolution for whole-brain fMRI applications in the macaque at 7T, 23rd Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2015), Toronto, Canada.
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Lowe S, Zaldivar D, Murayama Y, van Rossum M, Logothetis NK and Panzeri S (April-2015): Cortical dynamics across V1 laminae generate independent frequency channels encoding visual information, BNA 2015 Festival of Neuroscience, Edinburgh, UK.
Lowe SC, Zaldivar D, Murayama Y, van Rossum MCW, Logothetis NK and Panzeri S (November-18-2014): Different cortical layers in V1 encode different visual information in different frequency bands, 44th Annual Meeting of the Society for Neuroscience (Neuroscience 2014), Washington, DC, USA.
Bahmani H, Murayama Y, Logothetis NK and Keliris GA (October-2014): Binocular Flash Suppression in the Primary Visual Cortex of Anesthetized and Awake Macaques, 15th Conference of Junior Neuroscientists of Tübingen (NeNa 2014): The Changing Face of Publishing and Scientific Evaluation, Schramberg, Germany.
Löwe S, Murayama Y, Zaldivar D, Logothetis N and Panzeri S (July-7-2014): Quantification of the Laminar and Frequency Structure of Information in Primary Visual Cortex, 9th FENS Forum of Neuroscience, Milano, Italy.
Eschenko O, Besserve M, Murayama Y, Evrard H, Beyerlein M, Oeltermann A and Logothetis NK (November-13-2013): BOLD responses associated with hippocampal ripples in the rat brain, 43rd Annual Meeting of the Society for Neuroscience (Neuroscience 2013), San Diego, CA, USA.
Besserve M, Bartels A, Murayama Y and Logothetis NK (October-15-2012): Centrality of the Mammalian Functional Brain Network, 42nd Annual Meeting of the Society for Neuroscience (Neuroscience 2012), New Orleans, LA, USA.
Panzeri S, Mazzoni A, Kayser C, Murayama Y, Quian Quiroga R, Martinez J and Logothetis NK (November-2011): Neurons in primary visual cortex encode naturalistic visual information using multiple temporal scales, 41st Annual Meeting of the Society for Neuroscience (Neuroscience 2011), Washington, DC, USA.
Magri C, Schridde U, Panzeri S, Murayama Y and Logothetis NK (July-2011): Different LFP frequency bands convey complementary information about the BOLD signal, Twentieth Annual Computational Neuroscience Meeting (CNS*2011), Stockholm, Sweden, BMC Neuroscience, 12(Supplement 1) 124-125.
Mazzoni A, Kayser C, Murayama Y, Martinez J, Quiroga RQ, Logothetis NK and Panzeri S (July-2011): Local field potential phase and spike timing convey information about different visual features in primary visual cortex, Twentieth Annual Computational Neuroscience Meeting (CNS*2011), Stockholm, Sweden, BMC Neuroscience, 12(Supplement 1) 147-148.
Schridde U, Whittingstall K, Murayama Y and Logothetis NK (November-2010): Cross-frequency coupling of neural signals within and between early visual areas V1 and V2, 40th Annual Meeting of the Society for Neuroscience (Neuroscience 2010), San Diego, CA, USA.
Besserve M, Murayama Y, Schölkopf B, Logothetis NK and Panzeri S (November-2010): High frequency phase-spike synchronization of extracellular signals modulates causal interactions in monkey primary visual cortex, 40th Annual Meeting of the Society for Neuroscience (Neuroscience 2010), San Diego, CA, USA.
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Last updated: Monday, 22.05.2017