Contact

Patricia Pais

Address: Spemannstr. 41
72076 Tuebingen
Room number: 4.B.10
Phone: +49 7071 601 917
Fax: +49 7071 601 701
E-Mail: Patricia.Pais

 

Picture of Pais, Patricia

Patricia Pais

Position: PhD Student  Unit: Yu Scheffler

My current research focus is the multimodal characterization of coma in rats. I use electrophysiology, functional MRI, optogenetics and calcium imaging, together with behavioral assessment, to investigate the mechanisms of coma induction and recovery.

Development and characterization of the coma state in the rat brain.

 

vs.

 

Zebrafish as a target for high Magnetic Fields.

 

Figure 1. Zebrafish larvae at 2 days post fertilization.

2015-2018: Study of the rat brain during the coma state.

Coma is an unconscious state characterized by lack of wake-sleep cycles, and extremely low level of neuronal activity. Currently, coma is mainly reported in a clinical view. Most of coma studies are performed from chronic stage, usually several months, or even several years after the onset. The pathophysiology and treatment of coma remain elusive due to the lack of reliable coma models, especially in small animals.

 

Brainstem coma model in rats

This coma model is based on the focal damage to the arousal nuclei located along the ascending reticular activating system by means of brainstem ischemia induced with a vasoconstrictor. The model allows tracking the neurological recovery of animals during the acute phase of coma progression, a period that is usually not accessible in humans for sistematic study of the brain function, and provides a platform to test treatment strategies aiming at promoting consciousness.

Our first results showed that this brainstem coma is associated with an initial respiratory depression lasting approximately one hour, a time during which only limb reflexes are observed, at most, followed by gradual recovery of neurological function, assessed with a newly developed rat coma scale (Tuebingen-Boston Rat Coma Scale). The neurological score correlated with the electrophysiological signals from cortex, which became isoelectric after coma induction and evolved into burst-suppression patterns and a later emergence of slow waves. Resting state fMRI indicated a gradual increase of connectivity from the basal forebrain, basal ganglia and thalamus, as coma progressed, which may be associated with a certain participation of these networks in regulating the brain states.

We are currently investigating the mechanisms underlying coma induction and recovery with parallel calcium imaging and electrophysiology as well as optogenetics-fMRI studies.

TRAINING IN SCIENCE

From July 2014: PhD student at Max Planck for Biological Cybernetics - Graduate School of Neuroscience Tuebingen.

2013-2014: M.Sc in BIOMEDICAL ENGINEERING (60 ECTS) at Madrid Technical University (Spain). Master Final Project: In vitro neuronal network growing under the influence of a pulsed magnetic field.

2009-2013: B.Sc in MEDICAL BIOLOGY (240 ECTS) at Alcalá University (Spain).

2012-2013: Training on Neuroscience (30 ECTS) at the Graduate School of Neuroscience, Tübingen University.

 

CERTIFICATES

October 2014: FELASA B for animal testing.

 

SCIENTIFIC EXPERIENCE

From July 2014: Max Planck Institute for Biological Cybernetics, Tübingen (Germany), for the development of my PhD Thesis in Neuroscience. Working with high magnetic field MRI in the rat brain combining optogenetics and other techniques. Working in developmental biology of Zebrafish under high magnetic fields.

November-July2014: Centre for Biomedical Technologies, Madrid (Spain). Dissection of the locust brain, neurons culture and magnetic fields-dependent neurite outgrowth evaluation.

August 2013: Orthopaedic Institute and Tissue Bank, Santiago de Compostela (Spain). Osteoblasts culture and treatment of sponge bone from multi organ donors.

February-July 2013: Analytical Chemistry department of Alcalá University. Six months working for developing a new diagnostic technique for Galactosemia in urine samples, using a microfluidic chip.

June-July2012: Immunology department of Alcalá University. Two months working on the analysis of white blood cells and autophagy experiments using flow cytometry.

 

SCIENTIFIC AWARDS

Spanish Society for Neuroscience - SENC 2017, Alicante (Spain).

Poster presentation award (title: "Experimental model for coma research").

European Society for Molecular Imaging - ESMI 2015, Tuebingen (Germany).

Poster presentation award (title: "High magnetic field induced otolith fusion in Zebrafish larvae").

Merkle H , Schulz H , Singh A, Yu X and Pais Roldán P (June-3-2015) Abstract Talk: Sensing the high magnetic field: Fusion of otoliths in zebrafish larvae entails a hint , 23rd Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2015), Toronto, Canada23 (0695) .
Here we described the impact of the high magnetic field (MF) on zebrafish larvae aiming to identify potential biological MR sensors. 14T-MF exposures longer than 2 hours in zebrafish larvae led to fusion of 2 otoliths (CaCO3 crystals in the inner ear responsible for balance and hearing) and a subsequent aberrant balance behavior, a phenotype already described in genetic mutants. Identification of the cellular and molecular mechanisms underlying this MF-induced otolith-fusion may be tackled with a zebrafish mutagenesis approach and might contribute in an efficient way to search for MR sensors in biological models.
html CiteID: PaisRoldanSMSY2015

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Articles (1):

Pais-Roldán P, Singh AP, Schulz H and Yu X (April-2016) High magnetic field induced otolith fusion in the zebrafish larvae Scientific Reports 6(24151) 1-11.

Posters (4):

Pais P, Edlow B, Jiang Y, Zou M and Yu X (November-12-2017): A rat model of coma pathogenesis and recovery, 47th Annual Meeting of the Society for Neuroscience (Neuroscience 2017), Washington, DC, USA.
Chen Y, Pais-Roldán P, Chen X and Yu X (April-26-2017): Stepwise optogenetic activation of the rat thalamic nuclei with MRI-guided robotic arm (MgRA), 25th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2017), Honolulu, HI, USA.
Pais Roldán P, Edlow B and Yu X (May-9-2016): Developing a Rat Model of Brainstem Coma: Initial MRI and MRA Investigations of Basilar Artery Occlusion, 24th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2016), Singapore.
Pais Roldán P, Singh A, Merkle H, Schulz H and Yu X (March-19-2015): Magnetic field-induced otolith fusion of the zebrafish larvae, 10th Annual Meeting of the European Society for Molecular Imaging (EMIM 2015), Tübingen, Germany.

Talks (1):

Pais Roldán P, Singh A, Merkle H, Schulz H and Yu X (June-3-2015) Abstract Talk: Sensing the high magnetic field: Fusion of otoliths in zebrafish larvae entails a hint, 23rd Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2015), Toronto, Canada(0695).

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Last updated: Monday, 22.05.2017