Contact

Patricia Pais

Address: Spemannstr. 41
72076 Tuebingen
Room number: 4.B.10
Phone: +49 7071 601 917
Fax: +49 7071 601 701
E-Mail: Patricia.Pais

 

Picture of Pais, Patricia

Patricia Pais

Position: PhD Student  Unit: Yu Scheffler

The main projects I work on are (for a description of the projects go to the "projects" section):

 

1. Development and characterization of the coma state in the rat brain.

 

vs.

 

2. Zebrafish as a target for high Magnetic Fields.

 

Figure 1. Zebrafish larvae at 2 days post fertilization.

2015-2018: Study of the rat brain during the coma state.

Coma is an unconscious state characterized by lack of wake-sleep cycles, and extremely low level of neuronal activity. Currently, coma is mainly reported in a clinical view. Most of coma studies are performed from chronic stage, usually several months, or even several years after the onset. The pathophysiology and treatment of coma remain elusive due to the lack of reliable coma models, especially in small animals.

 

The aim of this project can be divided in two stages:

 

Build up a coma model in rats

This coma model consists of the induction of cardiac arrest (CA) by means of a time-controlled asphyxia. Asphyxial CA is a well-established method to produce anoxic brain injury in rats (1,2,3,4,5). This model will result in global ischemia, mimicking human drowning or choking scenarios.

 

The main steps for the CA induction are: 1. Anesthesia; 2. Tracheal intubation; 3. Neuro-muscular blocker; and 4. Disconnection of the ventilation (asphyxia). The time of the asphyxia will be adjusted to the comatose outcome.

 

The resuscitation from CA could be performed manually or remotely.

  • In the manual resuscitation, adrenaline is intravenously injected after reconnection of the ventilator and chest compressions are provided at 4s-1.
  • In the remote resuscitation (which allows to perform CA and resuscitation in a rat that lies in the bore of the magnet of an MRI), a special catheter advanced until the thoracic Aorta is needed. This catheter allows withdrawal and collection of oxygenated blood in a syringe containing adrenaline, before the asphyxia step, which will be infused directly into the thoracic Aorta for resuscitation.

 

Study the rat comatose brain

Study the brain, with special emphasis in arousal pathways. For this purpose, fMRI combined with optogenetics and EEG will be performed in the comatose rats.

 

With this project we aim to get solid knowledge about the coma condition in CA-coma-induced rats.

 

References:

(1): Callaway C.V. and Logue E.S. Asphyxial Cardiac Arrest. Animal models of Acute Nurological Injuries (8)

(2): Manole M.D. et al. Brain tissue oxygen monitoring identifies cortical hypoxia and thalamic hyperoxia after experimental pediatric cardiac arrest. Pediatr Res. 2014; 75(2): 295-301

(3): Shoykhet M. et al. Thalamocortical dysfunction  and thalamic injury after asphyxial cardiac arrest in developing rats. J Neurosci. 2012; 32(4): 4972-4981

(4): Jia et al. Quantitative EEG and neurological recovery with therapeutic hypothermia after asphyxial cardiac arrest in rats. Brain Res. 2006; 1111(1): 166-175

(5): Liachenko S. et al. A reproducible model of circulatory arrest and remote resuscitation in rats for NMR investigation. Stroke. 1998; 29: 1229-1239

2014-2016: Zebrafish as a target for High Magnetic Fields.

How high magnetic fields (MFs) interact with the biological processes remains ambiguous and sometimes neglected. Despite a diamagnetic feature dominating the animal or human body, some unintended biological interferences at the microscopic level in certain organs may occur under strong MFs. In recent years there have been several reports indicating the occurrence of vertigo and nystagmus experienced by human patients undergoing magnetic resonance imaging (MRI). These signs suggest a vulnerability for the rotational sensors to sense high MFs.

In this project we aim to investigate the effects of high MFs provided by MRI scanners in an animal model, paying special attention to the vestibular system. For this purpose we are working with zebrafish (Danio rerio), which is a genetically tractable model vertebrate, widely used because of its rapid and well-studied development, its small but manageable size and a relatively easy breeding.

Figure 1. Zebrafish larvae at 2 days post fertilization.

 

 

TRAINING IN SCIENCE

From July 2014: PhD student at Max Planck for Biological Cybernetics - Graduate School of Neuroscience Tuebingen.

2013-2014: M.Sc in BIOMEDICAL ENGINEERING (60 ECTS) at Madrid Technical University (Spain). Master Final Project: In vitro neuronal network growing under the influence of a pulsed magnetic field.

2009-2013: B.Sc in MEDICAL BIOLOGY (240 ECTS) at Alcalá University (Spain).

2012-2013: Training on Neuroscience (30 ECTS) at the Graduate School of Neuroscience, Tübingen University.

 

CERTIFICATES

October 2014: FELASA B for animal testing.

 

SCIENTIFIC EXPERIENCE

From July 2014: Working at Max Planck Institute for Biological Cybernetics, Tübingen (Germany), for the development of my PhD Thesis in Neuroscience. Working with high magnetic field MRI in the rat brain combining optogenetics and other techniques. Working in developmental biology of Zebrafish under high magnetic fields.

November-July2014: Training at Centre for Biomedical Technologies, Madrid (Spain). Dissection of the locust brain, neurons culture and magnetic fields-dependent neurite outgrowth evaluation.

August 2013: Basic training at Orthopaedic Institute and Tissue Bank, Santiago de Compostela (Spain). Osteoblasts culture and treatment of sponge bone from multi organ donors.

February-July 2013: Internship at the department of Analytical Chemistry of Alcalá University. Six months working for developing a new diagnostic technique for Galactosemia in urine samples, using a microfluidic chip.

June-July2012: Internship at the department of Immunology of Alcalá University. Two months working on the analysis of white blood cells and autophagy experiments using flow cytometry.

 

CONFERENCES

International Society for Magnetic Resonance in Medicine - ISMRM 2015, Toronto (Canada).

Oral presentation "Fusion of otoliths under the high Magnetic Field".

European Society for Molecular Imaging - ESMI 2015, Tuebingen (Germany).

Poster presentation award.

Bruker Preclinical Imaging Users' Meeting 2014, Ettlingen (Germany).

VI Workshop on Analytical Nanoscience and  Nanotechnology VINyNA 2013 (Spain). Member of the Organizing Committee.

ABSTRACTS/POSTERS:

June 2015: "Sensing the high magnetic field: Fusion of otoliths in zebrafish larvae entails a hint" at ISMRM (International Society for Magnetic Resonance in Medicine), Toronto, Canada.

March 2015: "Magnetic field-induced otolith fusion of the zebrafish larvae" at ESMI (European Society for Molecular Imaging), Tuebingen, Germany.

July 2013: "Portable microfluidic nano-device for early diagnosis of Galactosemia" at the VI Workshop on Analytical Nanoscience and Nanotechnology VINyNA, Madrid, Spain.

Merkle H , Schulz H , Singh A, Yu X and Pais Roldán P (June-3-2015) Abstract Talk: Sensing the high magnetic field: Fusion of otoliths in zebrafish larvae entails a hint , 23rd Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2015), Toronto, Canada23 (0695) .
Here we described the impact of the high magnetic field (MF) on zebrafish larvae aiming to identify potential biological MR sensors. 14T-MF exposures longer than 2 hours in zebrafish larvae led to fusion of 2 otoliths (CaCO3 crystals in the inner ear responsible for balance and hearing) and a subsequent aberrant balance behavior, a phenotype already described in genetic mutants. Identification of the cellular and molecular mechanisms underlying this MF-induced otolith-fusion may be tackled with a zebrafish mutagenesis approach and might contribute in an efficient way to search for MR sensors in biological models.
html CiteID: PaisRoldanSMSY2015

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Articles (1):

Pais-Roldán P, Singh AP, Schulz H and Yu X (April-2016) High magnetic field induced otolith fusion in the zebrafish larvae Scientific Reports 6(24151) 1-11.

Posters (4):

Pais P, Edlow B, Jiang Y, Zou M and Yu X (November-12-2017): A rat model of coma pathogenesis and recovery, 47th Annual Meeting of the Society for Neuroscience (Neuroscience 2017), Washington, DC, USA.
Chen Y, Pais-Roldán P, Chen X and Yu X (April-26-2017): Stepwise optogenetic activation of the rat thalamic nuclei with MRI-guided robotic arm (MgRA), 25th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2017), Honolulu, HI, USA.
Pais Roldán P, Edlow B and Yu X (May-9-2016): Developing a Rat Model of Brainstem Coma: Initial MRI and MRA Investigations of Basilar Artery Occlusion, 24th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2016), Singapore.
Pais Roldán P, Singh A, Merkle H, Schulz H and Yu X (March-19-2015): Magnetic field-induced otolith fusion of the zebrafish larvae, 10th Annual Meeting of the European Society for Molecular Imaging (EMIM 2015), Tübingen, Germany.

Talks (1):

Pais Roldán P, Singh A, Merkle H, Schulz H and Yu X (June-3-2015) Abstract Talk: Sensing the high magnetic field: Fusion of otoliths in zebrafish larvae entails a hint, 23rd Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2015), Toronto, Canada(0695).

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Last updated: Monday, 22.05.2017