This file was created by the Typo3 extension sevenpack version 0.7.14 --- Timezone: CEST Creation date: 2013-05-27 Creation time: 04-55-49 --- Number of references 1 article 6149 Proceedings of the National Academy of Sciences of the United States of America 2009 11 106 46 19557-19562 We describe a miniaturized head-mounted multiphoton microscope and its use for recording Ca2+ transients from the somata of layer 2/3 neurons in the visual cortex of awake, freely moving rats. Images contained up to 20 neurons and were stable enough to record continuously for >5 min per trial and 20 trials per imaging session, even as the animal was running at velocities of up to 0.6 m/s. Neuronal Ca2+ transients were readily detected, and responses to various static visual stimuli were observed during free movement on a running track. Neuronal activity was sparse and increased when the animal swept its gaze across a visual stimulus. Neurons showing preferential activation by specific stimuli were observed in freely moving animals. These results demonstrate that the multiphoton fiberscope is suitable for functional imaging in awake and freely moving animals. http://www.kyb.tuebingen.mpg.de http://www.kyb.tuebingen.mpg.de http://www.kyb.tuebingen.mpg.de Research Group Kerr http://www.pnas.org/content/early/2009/11/03/0903680106.full.pdf+html Biologische Kybernetik Max-Planck-Gesellschaft en 10.1073/pnas.0903680106 jsawJSawinski dhwDJWallace davidDSGreenberg SGrossmann WDenk jkerrJNDKerr