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Dr. Jörn Engelmann

Address:	Spemannstr. 41 72076 Tübingen
Room number:	4.B.09
Phone:	+49 7071 601 704
Fax:	+49 7071 601 702
E-Mail:	joern.engelmann

Jörn Engelmann

Position: Guest Scientist Unit: Scheffler

Projects
Curriculum Vitae
Publications

Targeted Contrast Agents for Magnetic Resonance Imaging

The goal of our group is to develop new cell-specific contrast agents which are either internalized or trapped selectively in or on the target cells for structural MRI studies or tracking of cells in vivo by MRI. Target cells should be selectively labeled in the presence of other cell types without the target structure by using specific characteristics of the target cells/tissues (e.g. over-expression of proteins/enzymes, surface markers, receptors, changes of microenvironment).

For this purpose, our small group of chemists and biologists/biochemists is working together on the synthesis and biological evaluation in vitro and in vivo probes.

Since for targeted probes in particular correct interaction/binding with the cellular target and their localization inside or on the cells is highly important, one main focus is the biological evaluation in cell culture models in vitro.

In this respect, of special interest for us are uptake/binding efficacy, intracellular localization, specificity of the interaction/accumulation, intracellular transport, and biocompatibility.

For an overview of our projects, please go to our project group web page

or to the personal home pages of

Dr. Aneta Keliris

Dr. Sven Gottschalk

Dr. Rajendra Joshi

1984-1990 Studies of Chemistry, University of Bremen, Germany

1990 Diploma thesis under the supervision of Prof. D. Leibfritz, University of Bremen, Germany

“NMR-spectroscopic studies of metabolism of P-388 lymphoma cells“

1990-1995 Ph.D. thesis under the supervision of Prof. D. Leibfritz, University of Bremen, Germany

“Studies of the effects of alkylphosphocholines with multinuclear NMR-spectroscopy“

1996-2000 Postdoctoral Research Scientist, Organic Chemistry (Prof. D. Leibfritz), University of Bremen, Germany

2000-2004 Research Scientist, Biochemical Laboratory of the Department of Conservative Dentistry, Periodontology and Preventive Dentistry (Prof. W. Geurtsen), Hannover Medical School, Germany

“Chemical-biological interactions of components of resin-modified filling materials on human primary cells of the oral cavity“

(September 2002 - Mai 2004 Deputy head of the Biochemical Laboratory)

2004-present Research Scientist, High-Field Magnetic Resonance Center, Max-Planck-Institute for Biological Cybernetics, Tübingen, Germany

“Development of intracellular targeted contrast agents for MRI”

Since 2005 Project Leader “Contrast Agent Development” (Chemical Biology)

Articles (19):
•	Vibhute SM , Engelmann J , Verbić T , Maier ME , Logothetis NK and Angelovski G (February-2013) Synthesis and characterization of pH-sensitive, biotinylated MRI contrast agents and their conjugates with avidin Organic & Biomolecular Chemistry 11(8) 1294-1305.
•	Joshi R , Feldmann V , Koestner W , Detje C , Gottschalk S , Mayer HA , Sauer MG and Engelmann J (January-2013) Multifunctional silica nanoparticles for optical and magnetic resonance imaging Biological Chemistry 394(1) 125–135.
•	Keliris A , Mamedov I , Hagberg GE , Logothetis NK , Scheffler K and Engelmann J (September-2012) A smart 19F and 1H MRI probe with self-immolative linker as a versatile tool for detection of enzymes Contrast Media and Molecular Imaging 7(5) 478–483.
•	Dhingra Verma K , Mishra A , Engelmann J , Beyerlein M , Maier ME and Logothetis NK (September-2012) Magnetic-Field-Dependent 1H Relaxivity Behavior of Biotin/Avidin-Based Magnetic Resonance Imaging Probes ChemPlusChem 77(9) 758–769.
•	Mishra A , Joshi R , Engelmann J and Logothetis NK (April-2012) Synthesis and in Vitro Evaluation of a Biotinylated Dextran-Derived Probe for Molecular Imaging ACS Chemical Neuroscience 3(4) 268–273.
•	Mamedov I , Engelmann J , Eschenko O , Beyerlein M and Logothetis NK (February-2012) Dual-functional probes towards in vivo studies of brain connectivity and plasticity Chemical Communications 48(22) 2755-2757.
•	Mishra A , Gottschalk S , Engelmann J and Parker D (January-2012) Responsive imaging probes for metabotropic glutamate receptors Chemical Science 3(1) 131-135.
•	Feldmann V , Engelmann J , Gottschalk S and Mayer HA (January-2012) Synthesis, Characterization and Examination of Gd[DO3A-hexylamine]-functionalized Silica Nanoparticles as Contrast Agent for MRI-Applications Journal of Colloid and Interface Science 366(1) 70-79.
•	Mishra A , Schüz A , Engelmann J , Beyerlein M , Logothetis NK and Canals S (October-2011) Biocytin-derived MRI contrast agent for longitudinal brain connectivity studies ACS Chemical Neuroscience 2(10) 578–587.
•	Sweidan K , Engelmann J , Joshi R , Mubarak MS and El-Abadelah MM (October-2011) Synthesis of Some Cyclic Methylene 1,3-Diaza Barbiturates Derivatives Letters in Organic Chemistry 8(8) 603-605.
•	Placidi MP , Engelmann J , Natrajan LS , Logothetis NK and Angelovski G (September-2011) An aryl-phosphonate appended macrocyclic platform for lanthanide based bimodal imaging agents Chemical Communications 47(41) 11534-11536.
•	Keliris A , Ziegler T , Mishra R , Pohmann R , Sauer M , Ugurbil K and Engelmann J (April-2011) Synthesis and Characterization of a Cell-Permeable Bimodal Contrast Agent Targeting β-Galactosidase Biorganic & Medicinal Chemistry 19(8) 2529-2540.
•	Jha D , Mishra R , Gottschalk S , Wiesmüller KH , Ugurbil K , Maier ME and Engelmann J (March-2011) CyLoP-1: A Novel Cysteine-Rich Cell-Penetrating Peptide for Cytosolic Delivery of Cargoes Bioconjugate Chemistry 22(3) 319-328.
•	Joshi R , Jha D , Su W and Engelmann J (January-2011) Facile synthesis of peptide nucleic acids and peptide nucleic acid-peptide conjugates on an automated peptide synthesizer Journal of Peptide Science 17(1) 8-13.
•	Henig J , Tóth É , Engelmann J , Gottschalk S and Mayer HA (June-2010) Macrocyclic Gd3+ Chelates Attached to a Silsesquioxane Core as Potential Magnetic Resonance Imaging Contrast Agents: Synthesis, Physicochemical Characterization, and Stability Studies Inorganic Chemistry 49(13) 6124-6138.
•	Joshi R , Mishra R , Pohmann R and Engelmann J (April-2010) MR contrast agent composed of cholesterol and peptide nucleic acids: Design, synthesis and cellular uptake Bioorganic and Medicinal Chemistry Letters 20(7) 2238-2241.
•	Mishra R , Su W , Pohmann R , Pfeuffer J , Sauer MG , Ugurbil K and Engelmann J (September-2009) Cell-Penetrating Peptides and Peptide Nucleic Acid-Coupled MRI Contrast Agents: Evaluation of Cellular Delivery and Target Binding Bioconjugate Chemistry 20(10) 1860-1868.
•	Su W , Mishra R , Pfeuffer J , Wiesmüller KH , Ugurbil K and Engelmann J (February-2007) Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid - cell- penetrating peptide conjugate Contrast Media and Molecular Imaging 2(1) 42-49.
•	Mishra A , Pfeuffer J , Mishra R , Engelmann J , Mishra A , Ugurbil K and Logothetis NK (April-2006) A New Class of Gd-based DO3A-ethylamine-derived Targeted Contrast Agents for MR and Optical Imaging. Bioconjugate Chemistry 17(3) 773-780.
Conference papers (1):
•	Joshi R , Mishra R , Su W and Engelmann J (September-2008) CPP or cholesterol conjugation to antisense PNA for cellular delivery In: Peptides 2008: Chemistry of Peptides in Life Science Technology and Medicine, 30th European Peptide Symposium (30 EPS), FIPS, Helsinki, Finland, 550-551.
Contributions to books (1):
•	Mishra A , Mishra R , Canals S , Logothetis NK , Beyerlein M , Engelmann J , Schüz A and Dhingra K : Biocytin-based contrast agents for molecular imaging: an approach to developing new in vivo neuroanatomical tracers for MRI, 181-204. In: Neuroimaging - Methods, (Ed) P. Bright, InTech, Rijeka, Croatia, (February-2012).
Posters (40):
•	Mamedov I , Engelmann J , Hagberg G , Eschenko O and Logothetis NK (May-2012): Development of multimodal imaging probes for neuroanatomical connectivity studies in vivo by means of MRI, 20th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2012), Melbourne, Australia.
•	Brud A , Mishra R , Engelmann J , Su W , Ziegler T and Ugurbil K (2011): Intracellular MR Contrast Agents with Enzymatically Cleavable Part for Molecular Imaging, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
•	Gottschalk S , Balla DZ , Pohmann R and Engelmann J (September-2010): Molecular imaging of pancreatic islets in mice with a newly developed beta-cell specific superparamagnetic contrast agent the ultra high filed of 16.4T, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan.
•	Gottschalk S , Jha D and Engelmann J (September-2010): Oxidative modification of the novel cysteine-rich cell penetrating peptide CyLoP-1 increases cytosolic delivery, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan.
•	Angelovski G , Mamdeov I , Engelmann J , Gottschalk S , Balla DZ , Pohmann R and Logothetis NK (September-2010): The Use of Gadolinium-based Contrast Agents for Molecular and Cellular MRI: Quantitative Considerations, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan.
•	Engelmann J , Henig J , Gottschalk S and Mayer HA (May-2010): Gadoxane: a Novel Degradable Silsesquioxane Based Macromolecular MRI Contrast Agent, ISMRM-ESMRMB Joint Annual Meeting 2010, Stockholm, Sweden.
•	Gottschalk S , Balla DZ , Pohmann R and Engelmann J (May-2010): Towards in vivo visualization of pancreatic beta-cells in the mouse: Molecular imaging at 16.4T, ISMRM-ESMRMB Joint Annual Meeting 2010, Stockholm, Sweden.
•	Joshi R , Mishra R and Engelmann J (September-2009): Comparison of a lipid- vs. peptide-based delivery system for intracellular targeted MR imaging probes, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada.
•	Jha D , Mishra R , Gottschalk S , Wiesmüller K-H , Ugurbil K and Engelmann J (September-2009): CyLoP-1, a new tool for the cytosolic delivery of intracellular targeted probes, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada.
•	Mishra R , Jha D , Gottschalk S , Ugurbil K and Engelmann J (September-2009): CyLoP-1: A novel cell penetrating peptide with remarkable cellular uptake for cytosolic targeting, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada.
•	Dhingra K , Mishra A , Engelmann J , Schüz A , Canals S , Pohmann R , Beyerlein M , Maier ME and Logothetis NK (September-2009): Development of Bio-responsive Contrast Agents for Magnetic Resonance Imaging: Potential Applications of a Novel Precursor, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada.
•	Mishra A , Dhingra K , Mishra R , Schüz A , Engelmann J , Logothetis NK and Canals S (September-2009): Development of New In Vivo Gd-based Neuroanatomical Tracers for Magnetic Resonance Imaging, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada.
•	Engelmann J , Jha D , Mishra R , Wiesmüller K-H and Ugurbil K (April-2009): Efficient Intracellular Delivery of an MR Imaging Probe by a Novel Cell Penetrating Peptide, 17th Annual Meeting of the International Society for Magnetic Resonance in Medicine (ISMRM 2009), Honolulu, HI, USA.
•	Mishra R , Su W , Brud A , Sauer MG , Pfeuffer J , Ugurbil K and Engelmann J (September-2008): Cell Penetrating Peptides Delivering Intracellular Targeted Agents for Molecular Imaging, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 14(S1) 154-155.
•	Joshi R , Mishra R , Su W and Engelmann J (September-2008): CPP or Cholesterol Conjugated Antisense PNA for Cellular Delivery, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 14(S1) 157.
•	Brud AJ , Mishra R , Ugurbil K , Ziegler T and Engelmann J (September-2008): Evaluation of an Intracellular Contrast Agent Targeting Beta-Galactosidase for Cellular Labeling, 2008 World Molecular Imaging Congress (WMIC), Nice, France.
•	Engelmann J , Jha D , Mishra R , Wiesmüller K-H and Ugurbil K (September-2008): Novel Cell Penetrating Peptide for the Cellular Delivery of Molecular Imaging Probes, 2008 World Molecular Imaging Congress (WMIC), Nice, France.
•	Jha D , Mishra R , Ugurbil K , Engelmann J and Wiesmüller K-H (September-2008): Novel Cysteine-rich Cell Penetrating Peptide: Efficient Uptake and Cytosolic Localization, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 30(S1) 157.
•	Jha D , Wiesmüller K-H , Mishra R , Ugurbil K and Engelmann J (June-2008): A Novel Cysteine Rich Cell Penetrating Peptide: Gateway to Cytosol, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
•	Mishra R , Su W , Sauer M , Pfeuffer J , Ugurbil K and Engelmann J (June-2008): Cell Penetrating Peptide Assisted Intracellular Transport of mRNA Targeting Magnetic Resonance Imaging Contrast Agents, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
•	Jha D , Wiesmüller K-H , Mishra R , Ugurbil K and Engelmann J (June-2008): Development of an efficient cysteine rich cell penetrating peptide by structure activity studies, 33rd FEBS Congress and 11th IUBMB Conference: "Biochemistry of Cell Regulation", Athens, Greece, FEBS Journal, 275(Supplement S1) 221.
•	Joshi R , Su W , Mishra R and Engelmann J (June-2008): Evaluation of Cholesterol Conjugation to Antisense PNA for Cellular Delivery, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
•	Mishra R , Jha D , Wiesmüller K-H , Ugurbil K and Engelmann J (June-2008): Novel Peptide Delivering Directly into the Cytosol: Prospective Tool For Intracellular Targeting, 33rd FEBS Congress and 11th IUBMB Conference: "Biochemistry of Cell Regulation", Athens, Greece, FEBS Journal, 275(Supplement S1) 172.
•	Mishra R , Su W , Pfeuffer J , Ugurbil K and Engelmann J (2008): Direct Cytosolic Delivery of Molecular Imaging Agents Using Covalent Conjugates of Pyrenebutyrate, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
•	Engelmann J , Su W , Brud A , Mishra R , Pfeuffer J and Ugurbil K (2008): Intracellular Targeted Agents for Molecular Imaging, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
•	Jha D , Su W , Mishra R , Engelmann J , Wiesmüller KH , Maier ME , Pfeuffer J and Ugurbil K (September-2007): Cell internalizing conjugates for MR/Optical Imaging: CPP cargo relationship affects uptake for optical and MR imaging techniques, AMI/SMI Joint Molecular Imaging Conference 2007, Providence, RI, USA.
•	Mishra R , Su W , Engelmann J , Sauer M , Pfeuffer J and Ugurbil K (September-2007): Targeted MR agent based on Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate: Endocytosis hinders targeting, AMI/SMI Joint Molecular Imaging Conference 2007, Providence, RI, USA.
•	Engelmann J , Su W , Mishra R , Pfeuffer J , Wiesmüller KH and Ugurbil K (May-2007): MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate, 2007 Joint Annual Meeting ISMRM-ESMRMB, Berlin, Germany.
•	Jha D , Wu S , Mishra R , Engelmann J , Wiesmüller K-H , Maier ME , Pfeuffer J and Ugurbil K (March-2007): Cell Penetrating Peptides and their conjugates for optical and MR imaging techniques, 8th German Peptide Symposium, Heidelberg, Germany.