Contact

Dr. Giuseppe Gambino

Address: Max-Planck-Ring 11
72076 Tübingen
Room number: 3.A.01
Phone: +49 7071 601 932
E-Mail: giuseppe.gambino

 

Picture of Gambino, Giuseppe, Dr.

Giuseppe Gambino

Position: Postdoctoral Fellow  Unit: Angelovski

Experiences:

  • From March 3rd 2010 to July 2nd 2014 – Università degli Studi del Piemonte Orientale “Amedeo Avogadro”, DiSIT, Alessandria.

 

Post-Doc Fellowship

Main Tasks: Synthesis, purification and characterization of organometallic complexes as contrast agents for Magnetic Resonance Imaging (MRI).

Education:

  • From November 1st 2010 to March 11th 2014 – Università degli Studi del Piemonte Orientale “Amedeo Avogadro”, DiSIT, Alessandria.

PhD  in Chemical Sciences

 

Main Tasks: Synthesis, purification and characterization of organometallic complexes as contrast agents for Magnetic Resonance Imaging (MRI). Organic synthesis (macrocyclic and multimeric ligands synthesis, nitro-group reductions, boronic acids functionalization, supramolecular interactions with HSA and β-cyclodextrins), purification and physical-chemical characterization of the obtained compounds for biomedical applications. Large use of mono- and bidimensional Nuclear Magnetic Resonance (NMR), 1H relaxometry, HPLC and mass spectrometry as characterization techniques. Writing reports and articles drafts to submit for publication.

PhD thesis:High-relaxivity systems and molecular imaging probes for Magnetic Resonance Imaging applications”.

 

 

  • From January to October 2010 - Università degli Studi del Piemonte Orientale “Amedeo Avogadro”, DiSIT, Alessandria.

Master Degree in Chemical Sciences  - 110/110.

 

Main subjects: Organic Chemistry, Bioorganic Chemistry, Inorganic Chemistry, Bioinorganic Chemistry.

  • From January to December 2009 - Università degli Studi del Piemonte Orientale “Amedeo Avogadro”, DiSIT, Alessandria.

I level Master in Chemical Sciences and Technologies.

Main subjects: Organic Chemistry, Bioorganic Chemistry, Inorganic Chemistry, Bioinorganic Chemistry.

 

 

  • From October 2005 to April 2009 - Università degli Studi del Piemonte Orientale “Amedeo Avogadro”, DiSIT, Alessandria.

Bachelor degree in Chemistry  - 101/110.

Main subjects: Organic Chemistry, Bioorganic Chemistry, Inorganic Chemistry, Bioinorganic Chemistry, Physical Chemistry, Analitical Chemistry.

Engelmann J , Logothetis NK , Mamedov I , Hagberg GE , Tei L, Botta M and Gambino G (December-2012) Abstract Talk: Development of multimodal imaging probes for neuroanatomical connectivity studies in vivo, Fifth Annual World Molecular Imaging Congress (WMIC 2012), Dublin, Ireland, Molecular Imaging and Biology14 (Supplement 2) S1901.
In order to understand the functional activity in the brain, a detailed knowledge of anatomical connections between different brain regions is of great importance. Unfortunately the requirement for highly invasive technologies precludes most studies in living subjects. In spite of the rapid progress in the field of animal research combined with the neuroimaging technology, currently applied techniques demonstrate the absence of a completive method which can be used for such studies. In contrast to conventional techniques, volume imaging with MRI-visible neuronal tracers provides a complete description of large-scale three-dimensional (3-D) networks. The recent attempts to conjugate the commonly used paramagnetic complex Gd-DOTA with classical neuroanatomical tracers such as cholera toxin subunit B (CTB) or biocytin demonstrated the high potential of such systems to act in noninvasive connectivity studies.1,2 Here we have developed efficient neuronal tracers that can allow a more complete investigation of the neuronal networks using MR and optical imaging techniques. Modified mono- or bis- Gd-AAZTA3,4 complexes acting as a MR reporter and tetramethylrhodamine as optical reporter were conjugated to Dextran (MW 10,000) which is used extensively in neuroanatomical research (Fig.1 up). This enables the investigation of neuroanatomical connectivity in the brain by both MR and optical imaging. Fluorescence microscopy of neuronal cells incubated in vitro with different concentrations of the tracer molecules clearly demonstrated their effective internalization and localization mainly within the cell body of the cells but also transport along the cellular processes. In vivo injection into the motor cortex (M1) of rat demonstrate signal enhancement in several well-known subcortical targets of M1, including the somatosensory cortex (S1) and Caudate putamen (CPu) confirming the high potential of the new contrast agents and their applicability for in vivo connectivity studies (Fig.1 down). Such systems can enable a diversity of new experimental studies on various neuroscientific issues associated with longitudinal research on brain plasticity and neurode- or regeneration.
html doi CiteID: mamedovEHLGTB2012

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Articles (5):

Gambino G, Tei L, Carniato F and Botta M (August-2016) Amphiphilic Ditopic Bis-Aqua Gd-AAZTA-like Complexes Enhance Relaxivity of Lipidic MRI Nanoprobes Chemistry: An Asian Journal 11(15) 2139–2143.
Vágner A, D'Alessandria C, Gambino G, Schwaiger M, Aime S, Maiocchi A, Tóth I, Baranyai Z and Tei L (February-2016) A rigidified AAZTA-like ligand as efficient chelator for 68Ga radiopharmaceuticals Chemistry Select 1(2) 163–171.
Gambino G, De Pinto S, Tei L, Cassino C, Arena F, Gianolio E and Botta M (February-2014) A new ditopic GdIII complex functionalized with an adamantyl moiety as a versatile building block for the preparation of supramolecular assemblies JBIC Journal of Biological Inorganic Chemistry 19(2) 133–143.
Ermelindo A, Gambino G and Tei L (November-2013) Synthesis of a mixed carboxylate–phosphinate AAZTA-like ligand and relaxometric characterization of its GdIII complex Tetrahedron Letters 54(47) 6378–6380.
Gambino G, Engelmann J, Tei L, Botta M, Logothetis NK and Mamedov I (September-2013) Multimodal contrast agents for in vivo neuroanatomical analysis of monosynaptic connections Biomaterials 34(29) 7135–7142.

Posters (1):

Savic T, Gambino G, Bokharaie V, Noori H, Logothetis NK and Angelovski G (September-13-2018): Monitoring of ischemic stroke with calcium-responsive MR contrast agents, 11th Annual World Molecular Imaging Congress (WMIC 2018), Seattle, WA, USA.

Talks (1):

Mamedov I, Engelmann J, Hagberg GE, Logothetis NK, Gambino G, Tei L and Botta M (December-2012) Abstract Talk: Development of multimodal imaging probes for neuroanatomical connectivity studies in vivo, Fifth Annual World Molecular Imaging Congress (WMIC 2012), Dublin, Ireland, Molecular Imaging and Biology, 14(Supplement 2) S1901.

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Last updated: Monday, 22.05.2017