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Dr. Yusuke Murayama

Adresse: Spemannstr. 38
72076 Tübingen
Raum Nummer: 253
Tel.: 07071 601 663
Fax: 07071 601 652
E-Mail: yusuke.murayama

 

Bild von Murayama, Yusuke, Dr.

Yusuke Murayama

Position: Wissenschaftler  Abteilung: Logothetis

Introduction

In our efforts study the brain simultaneously at different spatiotemporal scales we have long been developing and optimizing two powerful methodologies. These are first, in vivo connectivity based on MR-detectable paramagnetic transsynaptic tracers, such as manganese, e.g.(Saleem et al., 2002, Murayama et al., 2006, Eschenko et al., 2010), and second, direct electrical stimulation and fMRI combined (DES-fMRI) (Tolias et al., 2005, Logothetis et al., 2010). The latter permits the investigation of many different large-scale interactions, including the organization of projective fields, large-scale effects of local synaptic plasticity changes, and the spatiotemporal profile of neuromodulatory effects induced by diffuse ascending systems. Yet, our most recent pilot physiology-MRI study examining global signal propagation following electrical stimulation has demonstrated that DES most likely silences all cortical areas receiving direct input from the fibers that they excite (Logothetis et al., 2010). Together with results from previous electrophysiology studies, this finding is thought to reflect the synaptic organization of microcircuits and their response to an unnatural spatiotemporal input organization. Specifically, optimal operation of recurrent microcircuits is only ensured with spatio-temporally structured input patterns that respect synaptic delays. ES violates this principle and consequently silences the output of neocortex. To better understand feedforward signal propagation for spatiotemporally blurred inputs, we have conducted two more series of experiments.

The first study aimed to examine whether unstructured whole-field visual stimulation may to some extent resemble the pattern of signal. We used whole-field luminance flicker as stimulus and recorded from areas V1 and V2 during EPI MR imaging. In the second study we have started a systematic mapping of thalamus by local microstimulation and imaging. The aim of this investigation is to map cortico-subcortico-cortical pathways by means of ortho- and antidromic stimulation and try to understand the logic of the replication principle, namely the existence of a cortico-thalamo-cortical connection for any given cortico-cortical connection of two points, each in one cortical area. We stimulate the pulvinar but we also record spontaneous activity and study locally generated characteristic events that may be related to thalamo-cortical communication.

 

Methods

Recording and microstimulation hardware, including electrodes and microdrives, were developed at the Max Planck Institute for Biological Cybernetics. Recording chambers and head holders were positioned stereotaxically on the basis of individual, high-resolution MRI. Stimulation sites were selected so as to ensure reliable electrically evoked BOLD activation on the operculum of the brain. Visual stimuli (structured stimuli or whole-field luminance flicker) were presented binocularly, initially with our own custom-made MR-compatible visual stimulator. Electrical microstimulation was performed with a custom-built constant current source. Current amplitude, pulse duration, train duration and stimulation frequency were controlled digitally using our own TCL-based software and a QNX (Canada) real time operating system.

 

Results and Initial Conclusions

The stimulation results were presented in detail in a recent publication (Logothetis et al., 2010). The response to luminance flicker was surprising indeed. Figure 1 shows the maps and Figure 2 the temporal dynamic of the BOLD and neural responses to two types of stimulation: structured (counterphase pinwheel) and full-field luminance flicker. Striking and consistence was the patent deactivation of V2. V1 showed PBR in some animal and NBR in others. Physiological responses often showed continuation of stimulus-induced modulation, albeit with a strongly reduced baseline activity. It follows that unstructured, full-field luminance stimulations, just like the electrical stimulation, blurs the normal spatiotemporal input patterns in cortex and strong disrupts signal propagation. Our current experiments in thalamus yielded the first patterns of cortical activation (work in progress). Characteristic is the widespread activation that is expected given the parallel projections of this nucleus to the entire cortex.

 

BOLD responses to counter-phase and Full-Field Luminance Flicker

Figure 1: BOLD responses to counter-phase and Full-Field Luminance Flicker. The left plots shows response to counterphase flicker. As expected there is strong V1 and V2 activation accompanied by sustained negative BOLD (NBR) in non-stimulated areas.

 

Time course of BOLD and of neural responses to counter-phase and Full-Field Luminance Flicker.

Figure 2: Time course of BOLD and of neural responses to counter-phase and Full-Field Luminance Flicker. Top row shows responses to structured flicker. Red and Blue curves are BOLD responses in V1 and V2 respectively. Peristimulus histograms show multiunit responses in V1 (left) and V2 (right). Bottom row: same conventions but for full-field luminance flicker.

 

 

Collaborations

In the aforementioned studies I have been collaborating with Mark Augath, Josina Goense and Hellmut Merkle on MR in MR imaging, with Alexander Rauch and Nikos K. Logothetis in neuroscientific matters and data analysis, and with Axel Oeltermann in developing all the hardware required to perform the experiments (see details in publications).

 

References

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Artikel (25):

Bahmani H Person, Murayama Y Person, Logothetis NK Person und Keliris GA Person (Juni-2014) Binocular flash suppression in the primary visual cortex of anesthetized and awake macaques PLoS ONE . im Druck
Watanabe M Person, Bartels A Person, Macke JH Person, Murayama Y Person und Logothetis NK Person (November-2013) Temporal Jitter of the BOLD Signal Reveals a Reliable Initial Dip and Improved Spatial Resolution Current Biology 23(21) 2146–2150.
Logothetis NK Person, Eschenko O Person, Murayama Y Person, Augath M Person, Steudel T Person, Evrard HC Person, Besserve M Person und Oeltermann A Person (November-2012) Hippocampal-cortical interaction during periods of subcortical silence Nature 491(7425) 547–553.
Biessmann F Person, Murayama Y Person, Logothetis NK Person, Müller KR Person und Meinecke FC (Juli-2012) Improved decoding of neural activity from fMRI signals using non-separable spatiotemporal deconvolutions NeuroImage 61(4) 1031–1042.
Eschenko O Person, Evrard HC Person, Neves RM Person, Beyerlein M Person, Murayama Y Person und Logothetis NK Person (Februar-2012) Tracing of noradrenergic projections using manganese-enhanced MRI NeuroImage 59(4) 3252–3265.
Magri C Person, Schridde U Person, Murayama Y Person, Panzeri S Person und Logothetis NK Person (Januar-2012) The Amplitude and Timing of the BOLD Signal Reflects the Relationship between Local Field Potential Power at Different Frequencies Journal of Neuroscience 32(4) 1395-1407.
Murayama Y Person, Augath M Person und Logothetis NK Person (Dezember-2011) Activation of SC during electrical stimulation of LGN: retinal antidromic stimulation or corticocollicular activation? Magnetic Resonance Imaging 29(10) 1351-1357.
Sultan F , Augath M Person, Murayama Y Person, Tolias AS Person und Logothetis NK Person (Dezember-2011) esfMRI of the upper STS: further evidence for the lack of electrically induced polysynaptic propagation of activity in the neocortex Magnetic Resonance Imaging 29(10) 1374-1381.
Watanabe M Person, Cheng K , Murayama Y Person, Ueno K , Asamizuya T , Tanaka K und Logothetis NK Person (November-2011) Attention But Not Awareness Modulates the BOLD Signal in the Human V1 During Binocular Suppression Science 334(6057) 829-831.
Mamedov I Person, Canals S Person, Henig J , Beyerlein M Person, Murayama Y Person, Mayer HA , Logothetis NK Person und Angelovski G Person (Dezember-2010) In Vivo Characterization of a Smart MRI Agent That Displays an Inverse Response to Calcium Concentration ACS Chemical Neuroscience 1(12) 819-828.
Sultan F , Hamodeh S , Murayama Y Person, Saleem KS und Logothetis NK Person (Oktober-2010) Flat map areal topography in Macaca mulatta based on combined MRI and histology Magnetic Resonance Imaging 28(8) 1159-1164.
Murayama Y Person, Biessmann F Person, Meinecke FC , Müller K-R Person, Augath M Person, Oeltermann A Person und Logothetis NK Person (Oktober-2010) Relationship between neural and hemodynamic signals during spontaneous activity studied with temporal kernel CCA Magnetic Resonance Imaging 28(8) 1095-1103.
Logothetis NK Person, Augath M Person, Murayama Y Person, Rauch A Person, Sultan F , Goense J Person, Oeltermann A Person und Merkle H Person (Oktober-2010) The effects of electrical microstimulation on cortical signal propagation Nature Neuroscience 13(10) 1283-1291.
Eschenko O Person, Canals S Person, Simanova I , Beyerlein M Person, Murayama Y Person und Logothetis NK Person (Februar-2010) Mapping of functional brain activity in freely behaving rats during voluntary running using manganese-enhanced MRI: Implication for longitudinal studies Neuroimage 49(3) 2544-2555.
Logothetis NK Person, Murayama Y Person, Augath M Person, Steffen T Person, Werner J Person und Oeltermann A Person (Mai-2009) How not to study spontaneous activity NeuroImage 45(4) 1080-1089.
Canals S Person, Beyerlein M Person, Murayama Y Person und Logothetis NK Person (September-2008) Electric stimulation fMRI of the perforant pathway to the rat hippocampus Magnetic Resonance Imaging 26(7) 978-986.
Belitski A Person, Gretton A Person, Magri C Person, Murayama Y Person, Montemurro MA , Logothetis NK Person und Panzeri S Person (Mai-2008) Low-frequency Local Field Potentials and Spikes in Primary Visual Cortex Convey Independent Visual Information Journal of Neuroscience 28(22) 5696-5709.
Canals S Person, Beyerlein M Person, Keller AL Person, Murayama Y Person und Logothetis NK Person (April-2008) Magnetic Resonance Imaging of Cortical Connectivity in vivo Neuroimage 40(2) 458-472.
Rasch MJ Person, Gretton A Person, Murayama Y Person, Maass W und Logothetis NK Person (März-2008) Inferring Spike Trains From Local Field Potentials Journal of Neurophysiology 99(3) 1461-1476.
Montemurro MA , Rasch MJ Person, Murayama Y Person, Logothetis NK Person und Panzeri S Person (März-2008) Phase-of-Firing Coding of Natural Visual Stimuli in Primary Visual Cortex Current Biology 18(5) 375-380.
Gretton A Person, Belitski A Person, Murayama Y Person, Schölkopf B Person und Logothetis NK Person (April-2006) The Effect of Artifacts on Dependence Measurement in fMRI Magnetic Resonance Imaging 24(4) 401-409.
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Murayama Y Person, Weber B Person, Kadharbatcha SS , Augath M Person und Logothetis NK Person (März-2006) Tracing neural circuits in vivo with Mn-enhanced MRI Magnetic Resonance Imaging 24(4) 349-358.
Leopold DA Person, Murayama Y Person und Logothetis NK Person (April-2003) Very Slow Activity Fluctuations in Monkey Visual Cortex: Implications for Functional Brain Imaging Cerebral Cortex 13(4) 422-433.
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Wang Y , Fujita I , Tamura H und Murayama Y Person (2002) Contribution of GABAergic inhibition to receptive field structures of monkey inferior temporal cortex neurons. Cerebral Cortex 12 62-74.
Wang Y , Fujita I und Murayama Y Person (2000) Neuronal mechanisms of selectivity for object features revealed by blocking inhibition in inferotemporal cortex. Nature Neuroscience 3(8) 807-813.

Beiträge zu Tagungsbänden (2):

Biessmann F Person, Murayama Y Person, Logothetis NK Person, Müller K-R Person und Meinecke FC (2012) Non-separable Spatiotemporal Brain Hemodynamics Contain Neural Information In: Machine Learning and Interpretation in Neuroimaging, NIPS Workshop on Machine Learning and Interpretation in Neuroimaging (MLINI 2011), Springer, Berlin, Germany, 140-147.
Gretton A Person, Smola AJ Person, Bousquet O Person, Herbrich R , Belitski A Person, Augath M Person, Murayama Y Person, Pauls J Person, Schölkopf B Person und Logothetis NK Person (Januar-2005) Kernel Constrained Covariance for Dependence Measurement In: AISTATS 2005, Tenth International Workshop on Artificial Intelligence and Statistics (AI & Statistics 2005), 112-119.
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Poster (24):

Lowe S , Murayama Y Person, Zaldivar D Person, Logothetis N Person und Panzeri S Person (Juli-7-2014): Quantification of the Laminar and Frequency Structure of Information in Primary Visual Cortex, 9th FENS Forum of Neuroscience, Milano, Italy.
Eschenko O Person, Besserve M Person, Murayama Y Person, Evrard H Person, Beyerlein M Person, Oeltermann A Person und Logothetis NK Person (November-13-2013): BOLD responses associated with hippocampal ripples in the rat brain, 43rd Annual Meeting of the Society for Neuroscience (Neuroscience 2013), San Diego, CA, USA.
Besserve M Person, Bartels A Person, Murayama Y Person und Logothetis NK Person (Oktober-2012): Centrality of the Mammalian Functional Brain Network, 42nd Annual Meeting of the Society for Neuroscience (Neuroscience 2012), New Orleans, LA, USA.
Panzeri S Person, Mazzoni A , Kayser C Person, Murayama Y Person, Quian Quiroga R , Martinez J und Logothetis NK Person (November-2011): Neurons in primary visual cortex encode naturalistic visual information using multiple temporal scales, 41st Annual Meeting of the Society for Neuroscience (Neuroscience 2011), Washington, DC, USA.
Magri C Person, Schridde U Person, Panzeri S Person, Murayama Y Person und Logothetis NK Person (Juli-2011): Different LFP frequency bands convey complementary information about the BOLD signal, Twentieth Annual Computational Neuroscience Meeting (CNS*2011), Stockholm, Sweden, BMC Neuroscience, 12(Suppl 1) P204.
Mazzoni A , Kayser C Person, Murayama Y Person, Martinez J , Quiroga RQ , Logothetis NK Person und Panzeri S Person (Juli-2011): Local field potential phase and spike timing convey information about different visual features in primary visual cortex, Twentieth Annual Computational Neuroscience Meeting (CNS*2011), Stockholm, Sweden, BMC Neuroscience, 12(Suppl 1) P248.
Schridde U Person, Whittingstall K Person, Murayama Y Person und Logothetis NK Person (November-2010): Cross-frequency coupling of neural signals within and between early visual areas V1 and V2, 40th Annual Meeting of the Society for Neuroscience (Neuroscience 2010), San Diego, CA, USA.
Besserve M Person, Murayama Y Person, Schölkopf B Person, Logothetis NK Person und Panzeri S Person (November-2010): High frequency phase-spike synchronization of extracellular signals modulates causal interactions in monkey primary visual cortex, 40th Annual Meeting of the Society for Neuroscience (Neuroscience 2010), San Diego, CA, USA.
Mazzoni A , Kayser C Person, Murayama Y Person, Martinez J , Quiroga RQ , Logothetis NK Person und Panzeri S Person (Juni-2010): Primary Visual Cortex Encodes Complementary Information about Naturalistic Movies at Different Temporal Scales, AREADNE 2010: Research in Encoding And Decoding of Neural Ensembles, Santorini, Greece.
Biessmann F Person, Murayama Y Person, Meinecke FC , Logothetis NK Person und Müller K-R Person (Juni-2010): Spatio-Temporal Coupling between Neural Activity and Bold Response in Primary Visual Cortex, AREADNE 2010: Research in Encoding And Decoding of Neural Ensembles, Santorini, Greece.
Sultan FR , Augath M Person, Hamodeh S , Murayama Y Person, Thier P und Logothetis NK Person (Oktober-2009): Revealing polysynaptic propagation of excitation by microstimulation-fMRI of the deep cerebellar nuclei, 39th Annual Meeting of the Society for Neuroscience (Neuroscience 2009), Chicago, IL, USA.
Sultan F , Augath M Person, Murayama Y Person, Hamodeh S , Thier P und Logothetis NK Person (März-2009): Revealing Excitable Subcortical Networks by Microstimulationf-MRI of the Deep Cerebellar Nuclei, 8th Göttingen Meeting of the German Neuroscience Society, 32nd Göttingen Neurobiology Conference, Göttingen, Germany.
Sultan F , Murayama Y Person, Hamodeh S , Saleem KS und Logothetis NK Person (November-2008): A flattened representation of the neocortical areas of Macaca mulatta as obtained from combined MRI and histology, 38th Annual Meeting of the Society for Neuroscience (Neuroscience 2008), Washington, DC, USA.
Belitski A Person, Gretton A Person, Magri C Person, Murayama Y Person, Montemurro M , Logothetis NK Person und Panzeri S Person (November-2007): A time/frequency decomposition of information transmission by LFPs and spikes in the primary visual cortex, 37th Annual Meeting of the Society for Neuroscience (Neuroscience 2007), San Diego, CA, USA.
Canals S Person, Beyerlein M Person, Keller AL Person, Murayama Y Person und Logothetis NK Person (Mai-2007): In vivo brain connectivity: optimization of manganese enhanced MRI for neuronal tract tracing, 2007 Joint Annual Meeting ISMRM-ESMRMB, Berlin, Germany.
Canals S Person, Beyerlein M Person, Keller AL Person, Murayama Y Person und Logothetis NK Person (April-2007): In vivo brain connectivity: optimization of manganese enhanced MRI for neuronal tract tracing, 7th Meeting of the German Neuroscience Society, 31st Göttingen Neurobiology Conference, Göttingen, Germany, Neuroforum, 13(Supplement) 1221.
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Gretton A Person, Belitski A Person, Murayama Y Person, Schölkopf B Person und Logothetis NK Person (November-2005): Kernel methods for dependence testing in LFP-MUA, 35th Annual Meeting of the Society for Neuroscience (Neuroscience 2005), Washington, DC, USA.
Fust A , Pauls J Person, Augath M Person, Oeltermann A Person, Murayama Y Person und Logothetis NK Person (November-2003): The influence of anaesthetic agents on spiking and subthreshold activity in visual cortex revealed by electrophysiology and high-resolution functional MRI, 33rd Annual Meeting of the Society for Neuroscience (Neuroscience 2003), New Orleans, LA, USA.
Shmuel A Person, Augath MA Person, Oeltermann A Person, Pauls J Person, Murayama Y Person und Logothetis NK Person (Juni-2003): The Negative BOLD Response in Monkey V1 Is Associated with Decreases in Neuronal Activity, Ninth Annual Meeting of the Organization for Human Brain Mapping (OHBM 2003), New Orleans, LA, USA, NeuroImage, 19(2:Supplement) e567-e568.
Haiss F , Leopold DA Person, Murayama Y Person und Logothetis NK Person (Februar-2002): Dynamics of brain state transitions during anesthetic induction in the monkey, 5. Tübinger Wahrnehmungskonferenz (TWK 2002), Tübingen, Germany.
Leopold DA Person, Murayama Y Person und Logothetis NK Person (November-2001): Determinants of neural activity covariation in macaque visual cortex under different behavioral states, 31st Annual Meeting of the Society for Neuroscience (Neuroscience 2001), San Diego, CA, USA.
Murayama Y Person, Leopold DA Person und Logothetis NK Person (November-2001): Perception-related neural activity in the temporal cortex of monkeys under different conscious states, 31st Annual Meeting of the Society for Neuroscience (Neuroscience 2001), San Diego, CA, USA.
Leopold DA Person, Murayama Y Person und Logothetis NK Person (November-2000): Intra- and interareal covariation of neural activity during multistable perception in the monkey, 30th Annual Meeting of the Society for Neuroscience (Neuroscience 2000), New Orleans, LA, USA.
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Last updated: Freitag, 17.01.2014