Kontakt

Carsten Klein

Adresse: Spemannstr. 38
72076 Tübingen
Raum Nummer: 2.B.09
Tel.: 07071 601 695
Fax: 07071 601 652
E-Mail: carsten.klein

 

Bild von Klein, Carsten

Carsten Klein

Position: Doktorand  Abteilung: Logothetis

Investigation of thalamo-cortical connectivity in the primate brain with cell-targeted optogenetics and electrical micro-stimulation


The primate brain consists of various types and classes of cells that form anatomically segregated structures generally referred to as nuclei or areas which are highly interconnected within and between each other. Disentangling these connections on a functional and anatomical level is a major challenge in neuroscience. While anatomical tract tracing studies reveal the physical basis of neuronal connectivity within the brain, conclusions about their functional relevance are hard to derive from histological data alone. Likewise functional data obtained with electrophysiological recordings usually lacks the relevant underlying anatomical information. It is therefore desirable to combine anatomical and physiological data in order to unravel the functions of brain circuits.

 

In my work I investigate the connectivity of the lateral geniculate nucleus (LGN) with the primary visual cortex (V1) of the macaque monkey on a functional level with cell targeted optogenetics and electrical micro-stimulation. The LGN consist of three distinct neuronal subpopulations that relay information to V1, the magno-, parvo and konio cells. While much is known about the magno- and parvo-LGN cells the function of the konio cells is still not fully understood. Unlike the magno- and parvo-LGN cells which express the calcium binding protein parvalbumin konio LGN cells express another calcium binding protein called CamKIIα. This exclusive expression of CamKIIα within LGN konio neurons allows their selective manipulation through CamKIIα targeted optogenetics. In optogenetics neurons become responsive to direct light stimulation through the genetically enforced expression of light sensitive proteins called opsins. Therefore through the selective optogenetic stimulation of LGN konio cells their role in visual processing and their impact on V1 function can be studied in vivo.

 

The anatomical organization of the LGN into three segregated relay cell populations with little internal recurrence further offers the opportunity to directly compare the method of optogenetics to the less cell type specific method of electrical micro-stimulation. Results obtained in this project highlight the value of both stimulation techniques to investigate large scale brain circuits in primates.

 

see also:

http://neurosciencenews.com/visual-system-konio-neurons-optogenetics-4102/

 

http://www.cell.com/neuron/pdf/S0896-6273(16)00173-2.pdf

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Zeige Zusammenfassung

Artikel (2):

Klein C, Evrard HC, Shapcott KA, Haverkamp S, Logothetis NK und Schmid MC (April-2016) Cell-Targeted Optogenetics and Electrical Microstimulation Reveal the Primate Koniocellular Projection to Supra-granular Visual Cortex Neuron 90(1) 143–151.
Klein C, von der Behrens W und Gaese BH (Juli-2014) Stimulus-Specific Adaptation in Field Potentials and Neuronal Responses to Frequency-Modulated Tones in the Primary Auditory Cortex Brain Topography 27(4) 599-610.

Poster (4):

Klein C, Evrard HC, Shapcott K, Haverkamp S, Logothetis NK und Schmid MC (Oktober-19-2015): Functional identification of primate lateral geniculate nucleus projections to visual cortex using optogenetics and electrical stimulation, 45th Annual Meeting of the Society for Neuroscience (Neuroscience 2015), Chicago, IL, USA.
Klein C, Evrard HC, Shapcott K, Logothetis NK und Schmid MC (November-19-2014): Optogenetic investigation of the LGN koniocellular influence on V1 activity, 44th Annual Meeting of the Society for Neuroscience (Neuroscience 2014), Washington, DC, USA.
Klein C, Evrard HC, Power AT, Boyden ES, Logothetis NK und Schmid M (Oktober-2012): Optogenetics in the macaque thalamus, 42nd Annual Meeting of the Society for Neuroscience (Neuroscience 2012), New Orleans, LA, USA.
Klein C, Evrard HC, Power AT, Boyden ES, Logothetis NK und Schmid MC (Oktober-2012): Optogenetics in the macaque thalamus, 7th Brain Research Conference “Optogenetics and Pharmacogenetics in Neuronal Function and Dysfunction”, New Orleans, LA, USA.

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Last updated: Dienstag, 18.11.2014