Contact

Dr. Jörn Engelmann

Adresse: Spemannstr. 41
72076 Tübingen
Raum Nummer: 3.A.02
Tel.: 07071 601 704
Fax: 07071 601 702
E-Mail: joern.engelmann

 

Bild von Engelmann, Jörn, Dr.

Jörn Engelmann

Position: Wissenschaftstechnisches Personal  Abteilung: Alumni Scheffler

Targeted Contrast Agents for Magnetic Resonance Imaging and Their Biological Evaluation

The goal of our group has been the development of new cell-specific contrast agents that are either internalized or trapped selectively in or on the target cells for structural MRI studies or tracking of cells in vivo by MRI. Target cells should be selectively labeled in the presence of other cell types without the target structure by using specific characteristics of the target cells/tissues (e.g. over-expression of proteins/enzymes, surface markers, receptors, changes of microenvironment).

For this purpose, we perform synthesis and biological evaluation of such probes in vitro and in vivo.

 

Since for targeted probes in particular correct interaction/binding with the cellular target and their localization inside or on the cells is highly important, one main focus is the biological evaluation in cell culture models in vitro.

 

In this respect, of special interest for us are uptake/binding efficacy, intracellular localization, specificity of the interaction/accumulation, intracellular transport, and biocompatibility.

 

For an overview of recent or ongoing projects, please go also to our project group web page

or to the personal home pages of

 

Dr. Aneta Keliris

Dr. David Z. Balla

Dr. Sven Gottschalk

Dr. Rajendra Joshi

Für einen Überblick über unsere Projekte besuchen Sie bitte unsere Projektgruppen Webseite (momentan leider nur in Englisch).

1984-1990 Studium der Chemie, Universität Bremen, Deutschland

 

1990 Diplomarbeit unter der Leitung von Prof. D. Leibfritz, Universität Bremen, Deutschland

“NMR-spektroskopische Stoffwechseluntersuchungen an P-388 Lymphoma-Zellen“

 

1990-1995 Ph.D. thesis under the supervision of Prof. D. Leibfritz, Universität Bremen, Deutschland

“Untersuchungen zur Wirkungsweise von Alkylphosphocholinen mit Hilfe multinuklearer NMR-Spektroskopie“

 

1996-2000 Postdoktorand und wissenschaftlicher Mitarbeiter in der Abteilung von Prof. D. Leibfritz, Universität Bremen, Deutschland

 

2000-2004 Wissenschaftlicher Mitarbeiter, Biochemisches Laboratorium der Klinik für Zahnerhaltung, Parodontologie und Präventive Zahnheilkunde (Prof. W. Geurtsen), Medizinische Hochschule Hannover, Deutschland

“Chemisch-biologische Wechselwirkungen von Inhaltsstoffen Kunststoff-modifizierter Füllmaterialien mit humanen primären Zellen in der Mundhöhle“

(September 2002 - Mai 2004 Stellvertretender Leiter des Biochemischen Laboratoriums)

 

2004-2012 Wissenschaftlicher Mitarbeiter, Hochfeld Magnetresonanz, Max-Planck-Institute für biologische Kybernetik, Tübingen, Deutschland

“Entwicklung intrazellulär gezielter Kontrastmittel für die Magnetresonanz Bildgebung”

2005-2012 Projekt Leiter “Kontrastmittel-Entwicklung” (Chemische Biologie)

Seit 2012 Gastwissenschaftler

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Poster (59):

Joshi R, Feldmann V, Koestner W, Mayer HA, Sauer MG und Engelmann J (Juni-2011): Silica nanoparticles with multiple functionalities for optical and magnetic resonance imaging applications, 6th European Molecular Imaging Meeting (EMIM 2011), Leiden, The Netherlands.
Brud A, Mishra R, Engelmann J, Su W, Ziegler T und Ugurbil K (2011): Intracellular MR Contrast Agents with Enzymatically Cleavable Part for Molecular Imaging, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
Gottschalk S, Balla DZ, Pohmann R und Engelmann J (November-2010): Molecular imaging of pancreatic islets in mice with a newly developed beta-cell specific superparamagnetic contrast agent at the ultra high field of 16.4T, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan, Molecular Imaging and Biology, 12(Supplement 2) S750.
Gottschalk S, Jha D und Engelmann J (November-2010): Oxidative modification of the novel cysteine-rich cell penetrating peptide CyLoP-1 increases cytosolic delivery, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan, Molecular Imaging and Biology, 12(Supplement 2) S904.
Angelovski G, Mamdeov I, Engelmann J, Gottschalk S, Balla DZ, Pohmann R und Logothetis NK (November-2010): The Use of Gadolinium-based Contrast Agents for Molecular and Cellular MRI: Quantitative Considerations, 2010 World Molecular Imaging Congress (WMIC), Kyoto, Japan, Molecular Imaging and Biology, 12(Supplement 2) S768.
Engelmann J, Henig J, Gottschalk S und Mayer HA (Mai-6-2010): Gadoxane: a Novel Degradable Silsesquioxane Based Macromolecular MRI Contrast Agent, ISMRM-ESMRMB Joint Annual Meeting 2010, Stockholm, Sweden.
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Gottschalk S, Balla DZ, Pohmann R und Engelmann J (Mai-4-2010): Towards in vivo visualization of pancreatic beta-cells in the mouse: Molecular imaging at 16.4T, ISMRM-ESMRMB Joint Annual Meeting 2010, Stockholm, Sweden.
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Joshi R, Mishra R und Engelmann J (Februar-2010): Comparison of a lipid- vs. peptide-based delivery system for intracellular targeted MR imaging probes, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada, Molecular Imaging and Biology, 12(Supplement 1) S66.
Jha D, Mishra R, Gottschalk S, Wiesmüller K-H, Ugurbil K und Engelmann J (Februar-2010): CyLoP-1, a new tool for the cytosolic delivery of intracellular targeted probes, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada, Molecular Imaging and Biology, 12(Supplement 1) S33.
Mishra R, Jha D, Gottschalk S, Ugurbil K und Engelmann J (Februar-2010): CyLoP-1: A novel cell penetrating peptide with remarkable cellular uptake for cytosolic targeting, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada, Molecular Imaging and Biology, 12(Supplement 1) S83.
Dhingra K, Mishra A, Engelmann J, Schüz A, Canals S, Pohmann R, Beyerlein M, Maier ME und Logothetis NK (Februar-2010): Development of Bio-responsive Contrast Agents for Magnetic Resonance Imaging: Potential Applications of a Novel Precursor, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada, Molecular Imaging and Biology, 12(Supplement 1) S44.
Mishra A, Dhingra K, Mishra R, Schüz A, Engelmann J, Logothetis NK und Canals S (Februar-2010): Development of New In Vivo Gd-based Neuroanatomical Tracers for Magnetic Resonance Imaging, 2009 World Molecular Imaging Congress (WMIC), Montréal, Canada, Molecular Imaging and Biology, 12(Supplement 1) S51.
Mishra R, Jha D, Ugurbil K und Engelmann J (Mai-2009): Cylop-1 Peptide: a Tool for the Development of Targeted Intracellular Molecular Imaging Probes, 4th Europen Molecular Imaging Meeting (EMIM 2009), Barcelona, Spain.
Dhingra K, Mishra A, Engelmann J, Maier ME und Logothetis NK (Mai-2009): Development of MR guided probes for biotin/avidin based targeting techniques, 4th Europen Molecular Imaging Meeting (EMIM 2009), Barcelona, Spain.
Engelmann J, Jha D, Mishra R, Wiesmüller K-H und Ugurbil K (April-22-2009): Efficient Intracellular Delivery of an MR Imaging Probe by a Novel Cell Penetrating Peptide, 17th Annual Meeting of the International Society for Magnetic Resonance in Medicine (ISMRM 2009), Honolulu, HI, USA.
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Brud A, Mishra R, Ugurbil K, Ziegler T und Engelmann J (April-2009): Bimodal intracellular contrast agent based on the sugar moiety targeting beta-galactosidase, COST Chemistry D38 Annual Workshop: Metal-Based Systems for Molecular Imaging Applications, Warsaw, Poland.
Joshi R, Su W, Mishra R und Engelmann J (April-2009): Design, Synthesis and Relaxivity Behaviour of mRNA Targeting Contrast Agents, COST Chemistry D38 Annual Workshop: Metal-Based Systems for Molecular Imaging Applications, Warsaw, Poland.
Mishra R, Su W, Brud A, Sauer MG, Pfeuffer J, Ugurbil K und Engelmann J (September-2008): Cell Penetrating Peptides Delivering Intracellular Targeted Agents for Molecular Imaging, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 14(S1) 154-155.
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Joshi R, Mishra R, Su W und Engelmann J (September-2008): CPP or Cholesterol Conjugated Antisense PNA for Cellular Delivery, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 14(S1) 157.
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Brud AJ, Mishra R, Ugurbil K, Ziegler T und Engelmann J (September-2008): Evaluation of an Intracellular Contrast Agent Targeting Beta-Galactosidase for Cellular Labeling, 2008 World Molecular Imaging Congress (WMIC), Nice, France.
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Engelmann J, Jha D, Mishra R, Wiesmüller K-H und Ugurbil K (September-2008): Novel Cell Penetrating Peptide for the Cellular Delivery of Molecular Imaging Probes, 2008 World Molecular Imaging Congress (WMIC), Nice, France.
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Jha D, Mishra R, Ugurbil K, Engelmann J und Wiesmüller K-H (September-2008): Novel Cysteine-rich Cell Penetrating Peptide: Efficient Uptake and Cytosolic Localization, 30th European Peptide Symposium (30 EPS), Helsinki, Finland, Journal of Peptide Science, 30(S1) 157.
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Jha D, Wiesmüller K-H, Mishra R, Ugurbil K und Engelmann J (Juni-2008): A Novel Cysteine Rich Cell Penetrating Peptide: Gateway to Cytosol, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
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Mishra R, Su W, Sauer M, Pfeuffer J, Ugurbil K und Engelmann J (Juni-2008): Cell Penetrating Peptide Assisted Intracellular Transport of mRNA Targeting Magnetic Resonance Imaging Contrast Agents, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
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Jha D, Wiesmüller K-H, Mishra R, Ugurbil K und Engelmann J (Juni-2008): Development of an efficient cysteine rich cell penetrating peptide by structure activity studies, 33rd FEBS Congress and 11th IUBMB Conference: "Biochemistry of Cell Regulation", Athens, Greece, FEBS Journal, 275(Supplement S1) 221.
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Joshi R, Su W, Mishra R und Engelmann J (Juni-2008): Evaluation of Cholesterol Conjugation to Antisense PNA for Cellular Delivery, 2nd International Symposium "Cellular Delivery of Therapeutic Macromolecules 2008" (CDTM 2008), Cardiff, UK.
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Mishra R, Jha D, Wiesmüller K-H, Ugurbil K und Engelmann J (Juni-2008): Novel Peptide Delivering Directly into the Cytosol: Prospective Tool For Intracellular Targeting, 33rd FEBS Congress and 11th IUBMB Conference: "Biochemistry of Cell Regulation", Athens, Greece, FEBS Journal, 275(Supplement S1) 172.
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Mishra R, Su W, Pfeuffer J, Ugurbil K und Engelmann J (2008): Direct Cytosolic Delivery of Molecular Imaging Agents Using Covalent Conjugates of Pyrenebutyrate, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
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Engelmann J, Su W, Brud A, Mishra R, Pfeuffer J und Ugurbil K (2008): Intracellular Targeted Agents for Molecular Imaging, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
Jha D, Su W, Mishra R, Engelmann J, Wiesmüller KH, Maier ME, Pfeuffer J und Ugurbil K (September-2007): Cell internalizing conjugates for MR/Optical Imaging: CPP cargo relationship affects uptake for optical and MR imaging techniques, AMI/SMI Joint Molecular Imaging Conference 2007, Providence, RI, USA.
Mishra R, Su W, Engelmann J, Sauer M, Pfeuffer J und Ugurbil K (September-2007): Targeted MR agent based on Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate: Endocytosis hinders targeting, AMI/SMI Joint Molecular Imaging Conference 2007, Providence, RI, USA.
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Engelmann J, Su W, Mishra R, Pfeuffer J, Wiesmüller KH und Ugurbil K (Mai-21-2007): MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate, 2007 Joint Annual Meeting ISMRM-ESMRMB, Berlin, Germany.
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Jha D, Wu S, Mishra R, Engelmann J, Wiesmüller K-H, Maier ME, Pfeuffer J und Ugurbil K (März-2007): Cell Penetrating Peptides and their conjugates for optical and MR imaging techniques, 8th German Peptide Symposium, Heidelberg, Germany.
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Mishra R, Jha D, Su W, Engelmann J, Wiesmüller K-H, Maier ME, Pfeuffer J und Ugurbil K (2007): Cell internalizing conjugates for MR/Optical Imaging: CPP cargo relationship affects uptake, CMST COST Action D38: Metal-Based Systems for Molecular Imaging Applications, Tübingen, Germany.
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Engelmann J, Su W, Jha D, Mishra R, Pfeuffer J und Ugurbil K (Mai-9-2006): Intracellular MR Contrast Agents Based on Cationic Cell Penetrating Peptides: A Comparative Study, 14th Scientific Meeting of the International Society of Magnetic Resonance in Medicine (ISMRM 2006), Seattle, WA, USA.
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Mishra R, Engelmann J, Su W, Pfeuffer J und Ugurbil K (April-2006): Imaging Cells using Intracellular Bimodal Optical and MR contrast agents, COST Chemistry Action D18: Lanthanide Chemistry for Diagnosis and Therapy, Orléans, France.
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Brud A, Engelmann J, Pfeuffer J, Ziegler T und Ugurbil K (April-2006): Optical/MR Contrast Agents: Enzyme Activity and Relaxivity Studies, COST Chemistry Action D18: Lanthanide Chemistry for Diagnosis and Therapy, Orléans, France.
Jha D, Mishra R, Engelmann J, Pfeuffer J und Ugurbil K (April-2006): Peptide-coupled Intracellular Contrast Agents for Optical and MR Imaging, COST Chemistry Action D18: Lanthanide Chemistry for Diagnosis and Therapy, Orléans, France.
Jha D, Mishra R, Engelmann J, Mishra AK, Pfeuffer J und Ugurbil K (September-2005): Design and Synthesis of Peptide-coupled Intracellular Contrast Agents for Optical and MR Imaging, 4th Annual Meeting of the Society for Molecular Imaging (SMI 2005), Köln, Germany, Molecular Imaging, 4(3) 378.
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Brud A, Engelmann J, Pfeuffer J, Ziegler T und Ugurbil K (September-2005): Development and Synthesis of Optical/MR Contrast Agents for Detection of beta-Galactosidase Activity, 4th Annual Meeting of the Society for Molecular Imaging (SMI 2005), Köln, Germany, Molecular Imaging, 4(3) 378-379.
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Mishra R, Engelmann J, Su W, Pfeuffer J und Ugurbil K (September-2005): Intracellular MR Contrast Agents based on l-Tat and d-Tat: A Comparative Study, 4th Annual Meeting of the Society for Molecular Imaging (SMI 2005), Köln, Germany, Molecular Imaging, 4(3) 379.
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Su W, Mishra R, Engelmann J, Pfeuffer J und Ugurbil K (September-2005): Intracellular MR Contrast Agents based on l-Tat and d-Tat: A Comparative Study, COST Chemistry Action D18: Lanthanide Chemistry for Diagnosis and Therapy, Köln, Germany.
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Su W, Mishra R, Engelmann J, Pfeuffer J und Ugurbil K (September-2005): Preparation, Characterization and Visualization of CPP-Mediated MR Contrast Agents, 4th Annual Meeting of the Society for Molecular Imaging (SMI 2005), Köln, Germany, Molecular Imaging, 4(3) 378.
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Vorträge (10):

Wang M, He Y, Engelmann J und Yu X (September-2016) Abstract Talk: Decipher the neuronal and astrocytic calcium signal coupling with fMRI, 17th Conference of Junior Neuroscientists of Tübingen (NeNa 2016): Neuroscience & Law, Schramberg, Germany 12.
Engelmann J (September-10-2013) Invited Lecture: Potentials of NMR/MRS studies using stable isotopes and contrast agent enhanced MRI in diabetes research, Deutsches Diabetes-Zentrum (DDZ), Düsseldorf, Germany.
Keliris A, Hagberg GE, Engelmann J und Scheffler K (Mai-26-2013) Abstract Talk: Dual modality approach for detection of enzyme activity by means of 1H/19F MRI, 8th European Molecular Imaging Meeting (EMIM 2013), Torino, Italy(298).
Mamedov I, Engelmann J, Hagberg GE, Logothetis NK, Gambino G, Tei L und Botta M (November-2012) Abstract Talk: Development of multimodal imaging probes for neuroanatomical connectivity studies in vivo, Fifth Annual World Molecular Imaging Congress (WMIC 2012), Dublin, Ireland, Molecular Imaging and Biology, 14(Supplement 2) S1901.
Engelmann J (November-2011): Towards targeted MRI probes for in vivo applications, Symposium on MRI Probes for Molecular Imaging: From Design to Application, Tübingen, Germany.
Gottschalk S, Mishra A, Engelmann J und Parker D (Juni-19-2011) Abstract Talk: Responsive MR Imaging Probes to Monitor Synaptic Glutamate Fluctuations in the Brain, 6th European Molecular Imaging Meeting (EMIM 2011), Leiden, Netherlands 24.
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Balla D, Gottschalk S, Shajan G, Ueberberg S, Schneider S, Pohmann R und Engelmann J (Mai-12-2011) Abstract Talk: In vivo visualization of pancreatic islets in the mouse, 19th Annual Meeting and Exhibition of the International Society for Magnetic Resonance in Medicine (ISMRM 2011), Montréal, Canada 648.
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Last updated: Montag, 22.05.2017